anti dkk1 Search Results


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Bio-Rad dkk1
Upregulation of canonical WNT signaling in rHGP CRCLM. ( a ) Canonical WNT signaling pathway mapping: significant differentially expressed genes mapped according to the KEGG WNT signalling pathway. Colour coded according to scaled log fold change values. ( b ) Dot plot: significant differentially expressed genes regulated by LEF1. Mapped with expression percentages and colour coded according to scaled log fold change values. ( c ) GSEA: bulk sequencing data from CRCLM showing a gene signature regulated by LEF1 upregulated in rHGP ( n = 6 rHGP vs n = 9 dHGP). ( d ) IHC staining: <t>DKK1</t> (brown) in CRCLM ( n = 130, cut-off 80% angiogenic or vessel-coopting HGP). ( e ) Violin plot: quantification of d. ( f ) Scatter plot: TOP/FOP-Flash WNT reporter assay with conditioned media from PDOs ( n = 5 rHGP vs n = 3 dHGP). ( g ) Western blot: active and total ß-Catenin in protein lysates of PDOs ( n = 5 rHGP vs n = 5 dHGP). ( h ) Western blot quantification of g. ( i ) qRT- PCR analysis for DKK1 ( n = 6 rHGP vs n = 4 dHGP) and DKK4 ( n = 4 rHGP vs n = 5 dHGP) in PDO-derived RNA. ( j ) Bar plot: RT-PCR analysis for LRP6 ( n = 4 rHGP vs. n = 5 dHGP) in PDO-derived RNA. P values were calculated by unpaired t-test
Dkk1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio anti dkk 1
Upregulation of canonical WNT signaling in rHGP CRCLM. ( a ) Canonical WNT signaling pathway mapping: significant differentially expressed genes mapped according to the KEGG WNT signalling pathway. Colour coded according to scaled log fold change values. ( b ) Dot plot: significant differentially expressed genes regulated by LEF1. Mapped with expression percentages and colour coded according to scaled log fold change values. ( c ) GSEA: bulk sequencing data from CRCLM showing a gene signature regulated by LEF1 upregulated in rHGP ( n = 6 rHGP vs n = 9 dHGP). ( d ) IHC staining: <t>DKK1</t> (brown) in CRCLM ( n = 130, cut-off 80% angiogenic or vessel-coopting HGP). ( e ) Violin plot: quantification of d. ( f ) Scatter plot: TOP/FOP-Flash WNT reporter assay with conditioned media from PDOs ( n = 5 rHGP vs n = 3 dHGP). ( g ) Western blot: active and total ß-Catenin in protein lysates of PDOs ( n = 5 rHGP vs n = 5 dHGP). ( h ) Western blot quantification of g. ( i ) qRT- PCR analysis for DKK1 ( n = 6 rHGP vs n = 4 dHGP) and DKK4 ( n = 4 rHGP vs n = 5 dHGP) in PDO-derived RNA. ( j ) Bar plot: RT-PCR analysis for LRP6 ( n = 4 rHGP vs. n = 5 dHGP) in PDO-derived RNA. P values were calculated by unpaired t-test
Anti Dkk 1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio anti dkk1 antibody
Scutellarin Restored Podocyte Injury of the DN Mice. a Representative images of immunohistochemistry for NPHS1 and NPHS2 of the mice treated with vehicle, scutellarin or empagliflozin (× 200; scale bar = 50 µm). b Representative images of Western-blotting for NPHS1, NPHS2. c Quantitative plot of the expression of NPHS1 of the mice. d Quantitatification of NPHS1 expression of the mice. e Representative images of Western-blotting for β-catenin, Axin2, snail and <t>DKK1</t> of the mice. f – i Quantifications of the protein levels for β-catenin, Axin2, snail and DKK1 from E. All data are presented as the mean ± S.D.; n = 4–6 for each group, “n” stands for the number of animals; p vs. the model group (STZ)
Anti Dkk1 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Signalway Antibody rabbit monoclonal anti-dkk1 antibody #48979
Scutellarin Restored Podocyte Injury of the DN Mice. a Representative images of immunohistochemistry for NPHS1 and NPHS2 of the mice treated with vehicle, scutellarin or empagliflozin (× 200; scale bar = 50 µm). b Representative images of Western-blotting for NPHS1, NPHS2. c Quantitative plot of the expression of NPHS1 of the mice. d Quantitatification of NPHS1 expression of the mice. e Representative images of Western-blotting for β-catenin, Axin2, snail and <t>DKK1</t> of the mice. f – i Quantifications of the protein levels for β-catenin, Axin2, snail and DKK1 from E. All data are presented as the mean ± S.D.; n = 4–6 for each group, “n” stands for the number of animals; p vs. the model group (STZ)
Rabbit Monoclonal Anti Dkk1 Antibody #48979, supplied by Signalway Antibody, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cloud-Clone corp polyclonal anti-dkk-1 antibodies conjugated hrp
Scutellarin Restored Podocyte Injury of the DN Mice. a Representative images of immunohistochemistry for NPHS1 and NPHS2 of the mice treated with vehicle, scutellarin or empagliflozin (× 200; scale bar = 50 µm). b Representative images of Western-blotting for NPHS1, NPHS2. c Quantitative plot of the expression of NPHS1 of the mice. d Quantitatification of NPHS1 expression of the mice. e Representative images of Western-blotting for β-catenin, Axin2, snail and <t>DKK1</t> of the mice. f – i Quantifications of the protein levels for β-catenin, Axin2, snail and DKK1 from E. All data are presented as the mean ± S.D.; n = 4–6 for each group, “n” stands for the number of animals; p vs. the model group (STZ)
Polyclonal Anti Dkk 1 Antibodies Conjugated Hrp, supplied by Cloud-Clone corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Meso Scale Diagnostics LLC anti-dkk-1 humanized antibody calibration standards
Scutellarin Restored Podocyte Injury of the DN Mice. a Representative images of immunohistochemistry for NPHS1 and NPHS2 of the mice treated with vehicle, scutellarin or empagliflozin (× 200; scale bar = 50 µm). b Representative images of Western-blotting for NPHS1, NPHS2. c Quantitative plot of the expression of NPHS1 of the mice. d Quantitatification of NPHS1 expression of the mice. e Representative images of Western-blotting for β-catenin, Axin2, snail and <t>DKK1</t> of the mice. f – i Quantifications of the protein levels for β-catenin, Axin2, snail and DKK1 from E. All data are presented as the mean ± S.D.; n = 4–6 for each group, “n” stands for the number of animals; p vs. the model group (STZ)
Anti Dkk 1 Humanized Antibody Calibration Standards, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nature Biotechnology anti-dkk1 antibodies
Scutellarin Restored Podocyte Injury of the DN Mice. a Representative images of immunohistochemistry for NPHS1 and NPHS2 of the mice treated with vehicle, scutellarin or empagliflozin (× 200; scale bar = 50 µm). b Representative images of Western-blotting for NPHS1, NPHS2. c Quantitative plot of the expression of NPHS1 of the mice. d Quantitatification of NPHS1 expression of the mice. e Representative images of Western-blotting for β-catenin, Axin2, snail and <t>DKK1</t> of the mice. f – i Quantifications of the protein levels for β-catenin, Axin2, snail and DKK1 from E. All data are presented as the mean ± S.D.; n = 4–6 for each group, “n” stands for the number of animals; p vs. the model group (STZ)
Anti Dkk1 Antibodies, supplied by Nature Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ABclonal Biotechnology anti-dkk-1
Scutellarin Restored Podocyte Injury of the DN Mice. a Representative images of immunohistochemistry for NPHS1 and NPHS2 of the mice treated with vehicle, scutellarin or empagliflozin (× 200; scale bar = 50 µm). b Representative images of Western-blotting for NPHS1, NPHS2. c Quantitative plot of the expression of NPHS1 of the mice. d Quantitatification of NPHS1 expression of the mice. e Representative images of Western-blotting for β-catenin, Axin2, snail and <t>DKK1</t> of the mice. f – i Quantifications of the protein levels for β-catenin, Axin2, snail and DKK1 from E. All data are presented as the mean ± S.D.; n = 4–6 for each group, “n” stands for the number of animals; p vs. the model group (STZ)
Anti Dkk 1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ImmunoWay Biotechnology Company anti- dkk1 yt5562
Scutellarin Restored Podocyte Injury of the DN Mice. a Representative images of immunohistochemistry for NPHS1 and NPHS2 of the mice treated with vehicle, scutellarin or empagliflozin (× 200; scale bar = 50 µm). b Representative images of Western-blotting for NPHS1, NPHS2. c Quantitative plot of the expression of NPHS1 of the mice. d Quantitatification of NPHS1 expression of the mice. e Representative images of Western-blotting for β-catenin, Axin2, snail and <t>DKK1</t> of the mice. f – i Quantifications of the protein levels for β-catenin, Axin2, snail and DKK1 from E. All data are presented as the mean ± S.D.; n = 4–6 for each group, “n” stands for the number of animals; p vs. the model group (STZ)
Anti Dkk1 Yt5562, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Upregulation of canonical WNT signaling in rHGP CRCLM. ( a ) Canonical WNT signaling pathway mapping: significant differentially expressed genes mapped according to the KEGG WNT signalling pathway. Colour coded according to scaled log fold change values. ( b ) Dot plot: significant differentially expressed genes regulated by LEF1. Mapped with expression percentages and colour coded according to scaled log fold change values. ( c ) GSEA: bulk sequencing data from CRCLM showing a gene signature regulated by LEF1 upregulated in rHGP ( n = 6 rHGP vs n = 9 dHGP). ( d ) IHC staining: DKK1 (brown) in CRCLM ( n = 130, cut-off 80% angiogenic or vessel-coopting HGP). ( e ) Violin plot: quantification of d. ( f ) Scatter plot: TOP/FOP-Flash WNT reporter assay with conditioned media from PDOs ( n = 5 rHGP vs n = 3 dHGP). ( g ) Western blot: active and total ß-Catenin in protein lysates of PDOs ( n = 5 rHGP vs n = 5 dHGP). ( h ) Western blot quantification of g. ( i ) qRT- PCR analysis for DKK1 ( n = 6 rHGP vs n = 4 dHGP) and DKK4 ( n = 4 rHGP vs n = 5 dHGP) in PDO-derived RNA. ( j ) Bar plot: RT-PCR analysis for LRP6 ( n = 4 rHGP vs. n = 5 dHGP) in PDO-derived RNA. P values were calculated by unpaired t-test

Journal: Molecular Cancer

Article Title: Molecular differences of angiogenic versus vessel co-opting colorectal cancer liver metastases at single-cell resolution

doi: 10.1186/s12943-023-01713-1

Figure Lengend Snippet: Upregulation of canonical WNT signaling in rHGP CRCLM. ( a ) Canonical WNT signaling pathway mapping: significant differentially expressed genes mapped according to the KEGG WNT signalling pathway. Colour coded according to scaled log fold change values. ( b ) Dot plot: significant differentially expressed genes regulated by LEF1. Mapped with expression percentages and colour coded according to scaled log fold change values. ( c ) GSEA: bulk sequencing data from CRCLM showing a gene signature regulated by LEF1 upregulated in rHGP ( n = 6 rHGP vs n = 9 dHGP). ( d ) IHC staining: DKK1 (brown) in CRCLM ( n = 130, cut-off 80% angiogenic or vessel-coopting HGP). ( e ) Violin plot: quantification of d. ( f ) Scatter plot: TOP/FOP-Flash WNT reporter assay with conditioned media from PDOs ( n = 5 rHGP vs n = 3 dHGP). ( g ) Western blot: active and total ß-Catenin in protein lysates of PDOs ( n = 5 rHGP vs n = 5 dHGP). ( h ) Western blot quantification of g. ( i ) qRT- PCR analysis for DKK1 ( n = 6 rHGP vs n = 4 dHGP) and DKK4 ( n = 4 rHGP vs n = 5 dHGP) in PDO-derived RNA. ( j ) Bar plot: RT-PCR analysis for LRP6 ( n = 4 rHGP vs. n = 5 dHGP) in PDO-derived RNA. P values were calculated by unpaired t-test

Article Snippet: Samples were then incubated with the primary antibodies in an antibody diluent (Zytomed, ZUC025-100) with the following time periods and dilutions: LDHA (Thermo Fisher Scientific (TA500568), 1:200, 2 h); DKK1 (Bio-Rad (AHP1156) 1:100, 1 h), Ki67 (Invitrogen (MA5-14,520) 1:500, 1 h); Hif1α (Acris Antibodies (AP20633PU-N) 1:200, 1 h).

Techniques: Expressing, Sequencing, Immunohistochemistry, Reporter Assay, Western Blot, Quantitative RT-PCR, Derivative Assay, Reverse Transcription Polymerase Chain Reaction

Scutellarin Restored Podocyte Injury of the DN Mice. a Representative images of immunohistochemistry for NPHS1 and NPHS2 of the mice treated with vehicle, scutellarin or empagliflozin (× 200; scale bar = 50 µm). b Representative images of Western-blotting for NPHS1, NPHS2. c Quantitative plot of the expression of NPHS1 of the mice. d Quantitatification of NPHS1 expression of the mice. e Representative images of Western-blotting for β-catenin, Axin2, snail and DKK1 of the mice. f – i Quantifications of the protein levels for β-catenin, Axin2, snail and DKK1 from E. All data are presented as the mean ± S.D.; n = 4–6 for each group, “n” stands for the number of animals; p vs. the model group (STZ)

Journal: Natural Products and Bioprospecting

Article Title: Scutellarin ameliorates diabetic nephropathy via TGF-β1 signaling pathway

doi: 10.1007/s13659-024-00446-y

Figure Lengend Snippet: Scutellarin Restored Podocyte Injury of the DN Mice. a Representative images of immunohistochemistry for NPHS1 and NPHS2 of the mice treated with vehicle, scutellarin or empagliflozin (× 200; scale bar = 50 µm). b Representative images of Western-blotting for NPHS1, NPHS2. c Quantitative plot of the expression of NPHS1 of the mice. d Quantitatification of NPHS1 expression of the mice. e Representative images of Western-blotting for β-catenin, Axin2, snail and DKK1 of the mice. f – i Quantifications of the protein levels for β-catenin, Axin2, snail and DKK1 from E. All data are presented as the mean ± S.D.; n = 4–6 for each group, “n” stands for the number of animals; p vs. the model group (STZ)

Article Snippet: Scutellarin (Yunnan Phytopharmaceutical Co., LTD., China); Empagliflozin (Cat. C14295412, Macklin Biochemical, China), Streptozotocin (Cat. S8050, Solarbio, China); goat anti-rabbit immunoglobulin G (IgG) horseradish peroxidase (HRP)-linked antibody (Cat. AS014, Abclonal, China); anti-mouse IgG HRP-linked antibody (Cat. 7076S, Cell Signaling Technology, USA); Methenamine Silver Plating Stain Kit (Cat. G1790, Solarbio); Glycogen Periodic Acid Schiff (PAS) Stain Kit (Cat. G1281, Solarbio); Masson’s Trichrome Stain Kit (Cat. G1340, Solarbio); Mouse MAU enzyme-linked immunosorbent assay (ELISA) Kit (Cat. JL20493, JONIN, China); Anti-DKK1 antibody (Cat. A00632, Boster Biological Technology, China); Anti-SNAIl antibody (Cat. BP0449, Boster Biological Technology); Anti-α-SMA antibody (Cat. BM0002, Boster Biological Technology); Anti-TGFB1 antibody (Cat. BA0290, Boster Biological Technology); Anti-NPHS1 antibody (Cat. BA1669, Boster Biological Technology); Anti-NPHS2 antibody (Cat. BA1688, Boster Biological Technology); Anti-SMAD2/3 antibody (Cat. BA1395, Boster Biological Technology); Anti-Phospho-SMAD2(s250) antibody (Cat. BM4693, Boster Biological Technology); Anti-FN1 antibody (Cat. BA1772, Boster Biological Technology); AXIN2 antibody (Cat. Ab32197, Abcam, USA); Anti-Phospho-SMAD3(ser425) antibody (Cat. AF3362, Affinity Biosciences, China); Anti-COL3A1 antibody (Cat. M00788, Boster Biological Technology); Anti-extracellular signal-regulated kinase (ERK)1/2 antibody (Cat. ET1601-29, HUABIO, China); Anti-ERK1(PT202/PY204) + ERK2(PT185/PY187) antibody (Cat. ET1610-13, HUABIO); Anti-P38 antibody (Cat. ET1602-26, HUABIO); Anti-Phospho-P38(Thr180 + Tyr182) antibody (Cat. ER1903-01, HUABIO); Anti-β-Actin antibody (Cat. EM21002, HUABIO); Anti-SMAD4 antibody (Cat. A5657, Abclonal); Anti-β-catenin antibody (Cat. 610154, BD Biosciences, China); Creatinine (Cr) Assay kit (Cat. C011-2-1, Nanjing Jiancheng Bioengineering Institute, China); Glucose Assay Kit (Cat. F006-1-1, Nanjing Jiancheng Bioengineering Institute); microalbunminuria (MAU) ELISA kit (Cat. JL20493, JONIN).

Techniques: Immunohistochemistry, Western Blot, Expressing