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Image Search Results
Journal: Molecular Cancer
Article Title: Molecular differences of angiogenic versus vessel co-opting colorectal cancer liver metastases at single-cell resolution
doi: 10.1186/s12943-023-01713-1
Figure Lengend Snippet: Upregulation of canonical WNT signaling in rHGP CRCLM. ( a ) Canonical WNT signaling pathway mapping: significant differentially expressed genes mapped according to the KEGG WNT signalling pathway. Colour coded according to scaled log fold change values. ( b ) Dot plot: significant differentially expressed genes regulated by LEF1. Mapped with expression percentages and colour coded according to scaled log fold change values. ( c ) GSEA: bulk sequencing data from CRCLM showing a gene signature regulated by LEF1 upregulated in rHGP ( n = 6 rHGP vs n = 9 dHGP). ( d ) IHC staining: DKK1 (brown) in CRCLM ( n = 130, cut-off 80% angiogenic or vessel-coopting HGP). ( e ) Violin plot: quantification of d. ( f ) Scatter plot: TOP/FOP-Flash WNT reporter assay with conditioned media from PDOs ( n = 5 rHGP vs n = 3 dHGP). ( g ) Western blot: active and total ß-Catenin in protein lysates of PDOs ( n = 5 rHGP vs n = 5 dHGP). ( h ) Western blot quantification of g. ( i ) qRT- PCR analysis for DKK1 ( n = 6 rHGP vs n = 4 dHGP) and DKK4 ( n = 4 rHGP vs n = 5 dHGP) in PDO-derived RNA. ( j ) Bar plot: RT-PCR analysis for LRP6 ( n = 4 rHGP vs. n = 5 dHGP) in PDO-derived RNA. P values were calculated by unpaired t-test
Article Snippet: Samples were then incubated with the primary antibodies in an antibody diluent (Zytomed, ZUC025-100) with the following time periods and dilutions: LDHA (Thermo Fisher Scientific (TA500568), 1:200, 2 h);
Techniques: Expressing, Sequencing, Immunohistochemistry, Reporter Assay, Western Blot, Quantitative RT-PCR, Derivative Assay, Reverse Transcription Polymerase Chain Reaction
Journal: Natural Products and Bioprospecting
Article Title: Scutellarin ameliorates diabetic nephropathy via TGF-β1 signaling pathway
doi: 10.1007/s13659-024-00446-y
Figure Lengend Snippet: Scutellarin Restored Podocyte Injury of the DN Mice. a Representative images of immunohistochemistry for NPHS1 and NPHS2 of the mice treated with vehicle, scutellarin or empagliflozin (× 200; scale bar = 50 µm). b Representative images of Western-blotting for NPHS1, NPHS2. c Quantitative plot of the expression of NPHS1 of the mice. d Quantitatification of NPHS1 expression of the mice. e Representative images of Western-blotting for β-catenin, Axin2, snail and DKK1 of the mice. f – i Quantifications of the protein levels for β-catenin, Axin2, snail and DKK1 from E. All data are presented as the mean ± S.D.; n = 4–6 for each group, “n” stands for the number of animals; p vs. the model group (STZ)
Article Snippet: Scutellarin (Yunnan Phytopharmaceutical Co., LTD., China); Empagliflozin (Cat. C14295412, Macklin Biochemical, China), Streptozotocin (Cat. S8050, Solarbio, China); goat anti-rabbit immunoglobulin G (IgG) horseradish peroxidase (HRP)-linked antibody (Cat. AS014, Abclonal, China); anti-mouse IgG HRP-linked antibody (Cat. 7076S, Cell Signaling Technology, USA); Methenamine Silver Plating Stain Kit (Cat. G1790, Solarbio); Glycogen Periodic Acid Schiff (PAS) Stain Kit (Cat. G1281, Solarbio); Masson’s Trichrome Stain Kit (Cat. G1340, Solarbio); Mouse MAU enzyme-linked immunosorbent assay (ELISA) Kit (Cat. JL20493, JONIN, China);
Techniques: Immunohistochemistry, Western Blot, Expressing